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Citrullinated peptides and proteins can be detected using antibodies targeting the citrullinated residues, or detected using mass spectrometry-based proteomics technologies. Citrullination of arginine results in a monoisotopic mass increase of +0.984016 Da, which can be measured with mass spectrometry..
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測試版of protein citrullination remain challenging due to the difficulty of accessing proteins homogeneously citrullinated at a ... specific incorporation of unnatural amino acids (UAAs) into proteins ...
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When immune tolerance to citrullinated proteins is lost, anti-citrullinated proteins antibodies (ACPAs) are produced that recognize the newly formed epitopes []. ACPAs are detected in 75% of RA patients, and, therefore, their detection is used as a predictive method before disease onset as they are present in the serum of patients even 5 years prior to the symptomatic phase of the disease [ 3 ].
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測試版Peptide-bound arginine residues can undergo this modification and the resulting citrulline remains part of the protein as peptidyl citrulline. Citrulline is not a natural amino acid in proteins, therefore may induce immune response. The importance of citrullinated ...
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測試版Citrullinated proteins are implicated in autoimmune diseases. Understanding their causes and triggers is vital for developing treatments. Autoimmune Diseases Citrullination is the conversion of the amino acid arginine in a protein into the amino acid citrulline.
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測試版probability (>0.9) (Extended Data Fig. 2c), thereby ensuring high confidence in distinguishing between amino acids that become citrullinated or deamidated. The average localization probability of ...
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測試版The products of this process are citrullinated proteins, which contain a nonessential amino acid, citrulline. The immune system can recognize the deiminated or citrullinated proteins as nonself-antigens and so mount an autoimmune response.
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測試版Citrullinated proteins can trigger the production of anti-citrullinated protein antibodies (ACPAs), included in RA classification criteria. The immune response to citrullination involves both innate and adaptive immunity, affecting monocytes/macrophages, neutrophils, dendritic cells, natural killer cells, B cells, and T cells.
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測試版It is not known whether citrullinated vimentin and fibrin are just two of multiple citrullinated autoantigens in RA, or whether there is a dominant autoantigen that has yet to be described. The premise of the current study is that, if there were such a candidate, it is likely to be present in myeloid cells, the dominant cell type in the rheumatoid joint.
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測試版Citrullination induces both proximal and distal changes in processing relative to citrullinated sites in the linear amino acid sequence To investigate the relationship between citrulline residues ...
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Citrullinated peptides and proteins can be detected using antibodies targeting the citrullinated residues, or detected using mass spectrometry-based proteomics technologies. Citrullination of arginine results in a monoisotopic mass increase of +0.984016 Da, which can be measured with mass spectrometry..
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