Citrullination - Wikipedia

Citrullinated peptides and proteins can be detected using antibodies targeting the citrullinated residues, or detected using mass spectrometry-based proteomics technologies. Citrullination of arginine results in a monoisotopic mass increase of +0.984016 Da, which can be measured with mass spectrometry..

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Site-specific incorporation of citrulline into proteins in mammalian cells

of protein citrullination remain challenging due to the difficulty of accessing proteins homogeneously citrullinated at a ... specific incorporation of unnatural amino acids (UAAs) into proteins ...

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Citrullination in the pathology of inflammatory and autoimmune disorders: recent advances and future perspectives - PMC - PubMed Central (PMC)

When immune tolerance to citrullinated proteins is lost, anti-citrullinated proteins antibodies (ACPAs) are produced that recognize the newly formed epitopes []. ACPAs are detected in 75% of RA patients, and, therefore, their detection is used as a predictive method before disease onset as they are present in the serum of patients even 5 years prior to the symptomatic phase of the disease [ 3 ].

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Citrullination: A posttranslational modification in health and disease

Peptide-bound arginine residues can undergo this modification and the resulting citrulline remains part of the protein as peptidyl citrulline. Citrulline is not a natural amino acid in proteins, therefore may induce immune response. The importance of citrullinated ...

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Recent advances in characterization of citrullination and its implication in human disease research: From method development to network ...

Identification and characterization of citrullinated proteins is highly challenging, complicated by the low cellular abundance of citrullinated proteins, making it difficult to identify and quantify the extent of citrullination in samples, coupled with challenges associated

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Citrullinated Proteins: Unveiling The Underlying Causes And Triggers

Citrullinated proteins are implicated in autoimmune diseases. Understanding their causes and triggers is vital for developing treatments. Autoimmune Diseases Citrullination is the conversion of the amino acid arginine in a protein into the amino acid citrulline.

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A quantitative and site-specific atlas of the citrullinome reveals widespread existence of citrullination and insights into PADI4 substrates ...

probability (>0.9) (Extended Data Fig. 2c), thereby ensuring high confidence in distinguishing between amino acids that become citrullinated or deamidated. The average localization probability of ...

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Characterizing citrullination by mass spectrometry-based proteomics - PMC - PubMed Central (PMC)

Bennike T, Lauridsen KB, Olesen MK, Andersen V, Birkelund S, Stensballe A. 2013. Optimizing the identification of citrullinated peptides by mass spectrometry: utilizing the inability of trypsin to cleave after citrullinated amino acids. J. Proteom. Bioinform. 6. 288

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Insights into the complexities of Citrullination: From immune regulation to autoimmune disease - ScienceDirect

Citrullinated proteins can trigger the production of anti-citrullinated protein antibodies (ACPAs), included in RA classification criteria. The immune response to citrullination involves both innate and adaptive immunity, affecting monocytes/macrophages, neutrophils, dendritic cells, natural killer cells, B cells, and T cells.

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Citrullination modulates antigen processing and presentation by revealing cryptic epitopes in rheumatoid arthritis | Nature Communications

Amino acid positions with positive values are enriched in the PAD2- or PAD4-citrullinated (cit) samples compared to the native (nat) samples, while those with negative values are reduced by ...

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Citrullination

Citrullinated peptides and proteins can be detected using antibodies targeting the citrullinated residues, or detected using mass spectrometry-based proteomics technologies. Citrullination of arginine results in a monoisotopic mass increase of +0.984016 Da, which can be measured with mass spectrometry..

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